Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells |
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Authors: | Philip Heraud Elizabeth S. Ng Sally Caine Qing C. Yu Claire Hirst Robyn Mayberry Amanda Bruce Bayden R. Wood Don McNaughton Edouard G. Stanley Andrew G. Elefanty |
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Affiliation: | 1. Monash Immunology and Stem Cell Laboratories, Monash University, Building 75, STRIP 1, West Ring Road, Clayton, Victoria 3800, Australia;2. Centre for Biospectroscopy and the School of Chemistry, Monash University, Clayton, Victoria 3800, Australia |
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Abstract: | Human ESCs (hESCs) are a valuable tool for the study of early human development and represent a source of normal differentiated cells for pharmaceutical and biotechnology applications and ultimately for cell replacement therapies. For all applications, it will be necessary to develop assays to validate the efficacy of hESC differentiation. We explored the capacity for FTIR spectroscopy, a technique that rapidly characterises cellular macromolecular composition, to discriminate mesendoderm or ectoderm committed cells from undifferentiated hESCs. Distinct infrared spectroscopic “signatures” readily distinguished hESCs from these early differentiated progeny, with bioinformatic models able to correctly classify over 97% of spectra. These data identify a role for FTIR spectroscopy as a new modality to complement conventional analyses of hESCs and their derivatives. FTIR spectroscopy has the potential to provide low-cost, automatable measurements for the quality control of stem and differentiated cells to be used in industry and regenerative medicine. |
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