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Endothelial Cells Are Essential for the Self-Renewal and Repopulation of Notch-Dependent Hematopoietic Stem Cells
Authors:Jason M Butler  Daniel J Nolan  Eva L Vertes  Barbara Varnum-Finney  Hideki Kobayashi  Andrea T Hooper  Marco Seandel  Koji Shido  Ian A White  Mariko Kobayashi  Larry Witte  Chad May  Carrie Shawber  Yuki Kimura  Jan Kitajewski  Zev Rosenwaks  Irwin D Bernstein  Shahin Rafii
Institution:1. Howard Hughes Medical Institute, Department of Genetic Medicine and Ansary Stem Cell Institute, Weill Cornell Medical College, New York, NY 10065, USA;2. Department of Surgery, Weill Cornell Medical College, New York, NY 10065, USA;3. ImClone Systems Incorporated, 180 Varrick Street, New York, NY 10014, USA;4. Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, New York, NY 10065, USA;5. Department of OB/GYN, Columbia University Medical Center, New York, NY 10032, USA;6. Department of Pathology, Columbia University Medical Center, New York, NY 10032, USA;7. Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Abstract:
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  • Highlights? Coculture with endothelial cells promotes HSC expansion and self-renewal ? Endothelial cell effect on HSCs mediated by Notch signaling ? In vivo, sinusoidal endothelial cells (SECs) express Notch ligands ? Blocking vessel remodeling impairs hematopoietic recovery after irradiation
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