Image analysis of two-dimensional gel electrophoresis for comparative proteomics of transgenic and non-transgenic soybean seeds |
| |
| Authors: | A.R. Brandão H.S. Barbosa M.A.Z. Arruda |
| |
| Affiliation: | Spectrometry, Sample Preparation and Mechanization Group — GEPAM, Institute of Chemistry, University of Campinas — UNICAMP, P.O. Box 6154, 13083-970, Campinas, SP, Brazil;National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas — UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil;Universidade Nova de Lisboa, Caparica, Portugal;Universidade Nova de Lisboa, Caparica, Portugal;Universidade Nova de Lisboa, Caparica, Portugal |
| |
| Abstract: | Considering the importance of bidimensional electrophoresis and image analysis in comparative proteomics, the parameters that influence the analysis of protein expression of transgenic and non-transgenic soybean seeds were evaluated. The loaded mass of the proteins (150–500 µg), the pH separation range (3–10 or 4–7), and manual/automatic image editing were evaluated. Additionally, after optimizing the conditions, histograms and matchings were obtained in order to accurately analyze the variations (90%) in protein expression. From this, 10 proteins displayed significant differences in expression, and eight of them were characterized and identified by mass spectrometry. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
