A surface protein of Streptococcus suis serotype 2 identified by proteomics protects mice against infection |
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| Authors: | Francesca Mandanici Lidia Gómez-Gascón Manuela Garibaldi Alfonso Olaya-Abril Inmaculada Luque Carmen Tarradas Giuseppe Mancuso Salvatore Papasergi J Antonio Bárcena Giuseppe Teti Concetta Beninati Manuel J Rodríguez-Ortega |
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| Institution: | 1. The Elie Metchnikoff Department, University of Messina, Messina, Italy;2. Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain;3. Departamento de Sanidad Animal, Universidad de Córdoba, Córdoba, Spain;4. Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba, Spain |
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| Abstract: | Streptococcus suis serotype 2 is a major Gram-positive swine pathogen, causing also zoonoses. We describe here the immunoprotective activity in an in vivo animal model of a serotype-2 cell wall protein, designated Sat, which was identified by a previously validated proteomics approach consisting of the protease digestion of live bacteria and the selective recovery of exposed domains, followed by LC/MS/MS analysis. Increased survival rate (80%) and decreased bacterial burden were observed in mice immunized with a recombinant Sat fragment, suggesting that this protein is a potential vaccine candidate against serotype-2 infection. |
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