Preparation,Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging |
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Authors: | Derek J Averill Joel Garcia Buddhima N Siriwardena-Mahanama Sashiprabha M Vithanarachchi Matthew J Allen |
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Institution: | Department of Chemistry, Wayne State University |
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Abstract: | Polyaminopolycarboxylate-based ligands are commonly used to chelate lanthanide ions, and the resulting complexes are
useful as contrast agents for magnetic resonance imaging (MRI). Many commercially available ligands are especially useful because they contain
functional groups that allow for fast, high-purity, and high-yielding conjugation to macromolecules and biomolecules via amine-reactive
activated esters and isothiocyanate groups or thiol-reactive maleimides. While metalation of these ligands is considered common
knowledge in the field of bioconjugation chemistry, subtle differences in metalation procedures must be taken into account when
selecting metal starting materials. Furthermore, multiple options for purification and characterization exist, and selection of the most
effective procedure partially depends on the selection of starting materials. These subtle differences are often neglected in published
protocols. Here, our goal is to demonstrate common methods for metalation, purification, and characterization of lanthanide complexes
that can be used as contrast agents for MRI (Figure 1). We expect that this publication will enable biomedical scientists to incorporate
lanthanide complexation reactions into their repertoire of commonly used reactions by easing the selection of starting materials and
purification methods. |
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Keywords: | Medicine Issue 53 MRI contrast agent lanthanide gadolinium |
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