Crosstalk between blue-light- and aba-signaling pathways in stomatal guard cells |
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Authors: | Maki Hayashi Toshinori Kinoshita |
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Institution: | Division of Biological Science, Graduate School of Science, Nagoya University; Nagoya, Japan |
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Abstract: | We recently established an immunohistochemical method for the detection of blue light (BL)-induced and phototropin-mediated phosphorylation of plasma-membrane H+-ATPase in stomatal guard cells of Arabidopsis thaliana. This technique makes it possible to detect the phosphorylation/activation status of guard-cell H+-ATPase in the epidermis of a single rosette leaf, without the need to prepare guard-cell protoplasts (GCPs) from a large number of plants. Moreover, it can detect guard-cell responses under more natural and stress-free conditions compared to using GCPs. Taking advantage of these properties, we examined the effect of abscisic acid (ABA) on BL-induced phosphorylation of guard-cell H+-ATPase by using ABA-insensitive mutants. This revealed inhibition of BL-induced phosphorylation of guard-cell H+-ATPase via the early ABA-signaling components PYR/PYL/RCAR-PP2Cs-SnRK2s, which are known to be early ABA-signaling components for a wide range of ABA responses in plants. |
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Keywords: | ABA Blue light Guard cell Immunohistochemistry phosphorylation Plasma membrane H+-ATPase |
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