Isolation of C-phycocyanin from <Emphasis Type="Italic">Synechococcus</Emphasis> sp., (<Emphasis Type="Italic">Anacystis nidulans</Emphasis> BD1) |
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Authors: | Alka?Gupta Email author" target="_blank">Jayashree?K?SainisEmail author |
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Institution: | (1) Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India; |
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Abstract: | We report a procedure for obtaining fairly pure phycocyanin from a local isolate of the cyanobacterium Synechococcus sp (Anacystis nidulans BD1). Cells were incubated with 1 mg∙mL−1 of lysozyme at 37°C for 16 h with shaking. The cell-free extract was treated with activated charcoal and chitosan. The purity
(A
620/280) of phycocyanin obtained after lysozyme treatment was up to 2.18, which could be improved to 4.72 after incubation with activated
charcoal and chitosan. The yield of phycocyanin was 80–100 mg∙g−1 dry weight of cells. The method reported here is a single-step and efficient procedure and has the potential to be adopted
for large-scale production of phycocyanin. |
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Keywords: | |
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