Stable maintenance in chemostat-grownEscherichia coli of pBR322 and pACYC184 by disruption of the tetracycline resistance gene |
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| Authors: | Thierry Vernet Ian J McDonald Dave R Cameron Louis P Visentin |
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| Institution: | (1) Molecular Genetics Section, Division of Biological Sciences, National Research Council of Canada, K1A 0R6 Ottawa, Ontario, Canada;(2) Microbiology Section, Division of Biological Sciences, National Research Council of Canada, K1A 0R6 Ottawa, Ontario, Canada;(3) Department of Microbiology and Immunology, McGill University, 3775 University Street, H3A 2B4 Montreal, Quebec, Canada;(4) Montreal Biotechnology Research Institute, c/o National Research Council of Canada, K1A 0R6 Ottawa, Ontario, Canada |
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| Abstract: | Plasmid stability was studied in antibiotic-free chemo-stat cultures . Disruption, either by deletion or insertion, of the tetracycline resistance gene in the EcoRl/EcoRV region of the cloning vector pBR322 or in the HindIII]BamHl region of pACYCI84 yields plasmids markedly more stable than the parent plasmids. Thus, at least for these two instances, cloning of a partitioning (par) locus is not prerequisite for plasmid maintenance.Issued as NRCC publication No. 23992. |
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