ThPOD3, a truncated polypeptide from <Emphasis Type="Italic">Tamarix hispida</Emphasis>, conferred drought tolerance in <Emphasis Type="Italic">Escherichia coli</Emphasis> |
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Authors: | Xiao-Hong Guo Jing Jiang Bai-Chen Wang Hui-Yu Li Yu-Cheng Wang Chuan-Ping Yang Gui-Feng Liu |
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Institution: | (1) Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China; |
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Abstract: | The ThPOD1 gene encodes a peroxidase and was isolated from a Tamarix hispida NaCl-stress root cDNA library. We found that ThPOD1 expression could be induced by abiotic stresses such as cold, salt, drought and exogenous abscisic acid. These findings suggested
that ThPOD1 might be involved in the plant response to environmental stresses and ABA treatment. To elucidate the function of this gene,
recombinant plasmids expressing full-length ThPOD1 as well as ThPOD2 (aa 41-337), and ThPOD3 (aa 73-337) truncated polypeptides were constructed. SDS–PAGE and Western blot analyses of the fusion proteins revealed that
the molecular weights of ThPOD1, ThPOD2 and ThPOD3 were ~57, ~50 and ~47 kDa, respectively. Stress assays of E. coli treated with the recombinant plasmids indicated that ThPOD3 could improve resistance to drought stress. This finding could
potentially be used to improve plant tolerance to drought stress via gene transfer. |
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