| 40个黄皮品种的ISSR分析 |
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| 引用本文： | 李开拓,钟凤林,郭志雄,潘腾飞,王江波,潘东明. 40个黄皮品种的ISSR分析[J]. 亚热带植物科学, 2009, 38(4): 22-26. DOI: 10.3969/j.issn.1009-7791.2009.04.007 |
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| 作者姓名： | 李开拓 钟凤林 郭志雄 潘腾飞 王江波 潘东明 |
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| 作者单位： | 福建农林大学 园艺学院, 福建 福州 350002 |
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| 基金项目： | 台湾农业新品种、新技术引进创新研究与示范，台湾果树新品种与品质控制新技术引进创新研究 |
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| 摘 要： | 采用ISSR-PCR分子标记技术对40个黄皮品种的遗传多样性进行分析。从96条ISSR引物中筛选出15条引物用于PCR扩增,共扩增出165条带,其中多态性条带100条,多态性比率为60.6%。应用SPSS软件计算各品种间的Jaccard相似系数介于0.714~1.000,UPGMA法将40个品种分成5组。
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| 关 键 词： | 黄皮 ISSR 遗传关系 |
| 收稿时间： | 2009-08-07 |
ISSR Analysis of 40 Wampee Cultivars |
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| LI Kai-tuo,ZHONG Feng-lin,GUO Zhi-xiong,PAN Teng-fei,WANG Jiang-bo,PAN Dong-ming. ISSR Analysis of 40 Wampee Cultivars[J]. Subtropical Plant Science, 2009, 38(4): 22-26. DOI: 10.3969/j.issn.1009-7791.2009.04.007 |
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| Authors: | LI Kai-tuo ZHONG Feng-lin GUO Zhi-xiong PAN Teng-fei WANG Jiang-bo PAN Dong-ming |
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| Affiliation: | College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China |
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| Abstract: | ISSR-PCR markers were used to analyze the genetic diversity of 40 wampee cultivars.A total of 15 ISSR primers selected from 96 primers were used to amplify 165 DNA fragments and of which 100 (60.6%) were polymorphic loci.Based on SPSS 13.0 analysis, the similarity coefficient between different cultivars ranged from 0.714 to 1.000.The dendrogram constructed by UPGMA showed that 40 wampee cultivars were classified into five groups. |
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| Keywords: | ISSR Clausena lansium ISSR genetic relationship |
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