Esterase-29 (ES-29): Biochemical characterization and control by two independent gene loci of a testosterone-dependent mouse serum esterase |
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Authors: | Otto von Deimling Andreas Gaa |
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Institution: | (1) Pathologisches Institut, Abteilung für Chemische Pathologie, Universität Freiburg, D-7800 Freiburg i.B., BRD |
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Abstract: | Biochemistry and genetics of a testosterone-dependent murine serum esterase designated esterase-29 (ES-29) are described. The enzyme was identified after disc electrophoresis and subsequent staining for esterase using -naphthyl acetate as the substrate. It was inhibited by bis-p-nitrophenyl phosphate and was resistant top-chlorophenylsulphonate and hence was classified as carboxylesterase EC 3.1.1.1. The molecular mass was estimated to be about 130 kDa. It was shown that ES-29 is under the control of two independent genes. The first, termed Es-29, is suggested to be a structural locus, linked to the cluster-2 esterase loci on chromosome 8. Three alleles atEs-29, Es-29
a, Es-29b, andEs-29
care distinguished, which determine absence (SEG/1), strong activity (BALB/cJ), and low activity (MOLH/Fre), respectively. The second locus, termedMse-1 (serum esterase modifying factor), was found to be closely linked toPre-2 on chromosome 12 and is suggested to be a modifying or regulatory gene. Two alleles were distinguished,Mse-1
a(BALB/cJ) andMse-1
m(MOL3/JA, CasBgr), which determine whether ES-29 appears as a single band or a double band, respectively.Mse-1
mis dominant toMse-1
a.This work was supported by the Deutsche Forschungsgemeinschaft. This is communication No. 70 of a research program devoted to the cellular distribution, genetics, and regulation of nonspecific esterases. |
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Keywords: | Mus esterase-29 serum esterase modifying factor linkage |
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