Molecular methods of analysing bacterial diversity: the case of Rhizobia |
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| Authors: | Hiroshi Oyaizu Naohiro Naruhashi Takuma Gamou |
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| Affiliation: | (1) Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, 1-1-1 Yayoi, Bunkyoku, 113 Tokyo, Japan;(2) Department of Biology, Toyama University, Gofuku 3190, 930 Toyama, Japan;(3) National Institute of Agrobiological Resources, Tsukuba, Ibaraki, Japan |
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| Abstract: | The distribution of rhizobia, bacteria which nodulate the roots of leguminous plants, was surveyed for 91 species of the familyLeguminosae. A total of 117 strains of rhizobia were isolated, and 28 strains were obtained from culture collections. The sample total of 145 strains was discriminated by rapid ribosomal RNA (rRNA) sequencing. The partial sequences (157 bases from position (inEscherichia coli) 1220 to 1377 from 5 end) of 16S rRNA revealed the presence of 16 groups in these rhizobia. Further, DNA-DNA homology studies suggested that the differences of the 16 groups were enough to justify establishing at least 16 species. |
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| Keywords: | 16S rRNA Rhizobium Bradyrhizobium bacterial diversity DNA-DNA homology |
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