Evaluation of immunogenicity and protective efficacy of a Microsporum canis metalloprotease subunit vaccine in guinea pigs |
| |
Authors: | Vermout Sandy M Brouta Frédéric D Descamps Frédéric F Losson Bertrand J Mignon Bernard R |
| |
Affiliation: | Department of Infectious and Parasitic Diseases, Parasitology, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium. |
| |
Abstract: | In order to identify protective immunogens against Microsporum canis infection, a purified recombinant keratinolytic metalloprotease (r-MEP3) was tested as a subunit vaccine in experimentally infected guinea pigs. Both humoral and cellular specific immune responses developing towards r-MEP3 were evaluated, by enzyme-linked immunosorbent assay and by in vitro lymphocyte transformation tests respectively. Vaccination induced a strong antibody response, and a significant but transient lymphoproliferative response against the protein. However, the protocol failed to prevent fungal invasion or development of dermatophytic lesions. These results show that under the present experimental conditions, r-MEP3 specific antibodies are not protective against a challenge exposure. They also suggest that in the same model, the induction of cell-mediated immunity towards r-MEP3 is not sufficient, indicating the need for further research in the field of specific immune mechanisms involved in M. canis dermatophytosis. |
| |
Keywords: | Dermatophyte Dermatophyte vaccine Microsporum canis Metalloprotease |
本文献已被 PubMed 等数据库收录! |
|