Sulfated hyaluronan derivatives reduce the proliferation rate of primary rat calvarial osteoblasts |
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| Authors: | Reiner Kunze Manuela Rösler Stephanie Möller Matthias Schnabelrauch Thomas Riemer Ute Hempel Peter Dieter |
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| Institution: | 1.Institute of Physiological Chemistry,Dresden University of Technology,Dresden,Germany;2.Biomaterials Department,INNOVENT e.V.,Jena,Germany;3.Institute of Medical Physics and Biophysics,University Leipzig,Leipzig,Germany |
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| Abstract: | Glycosaminoglycans (GAG) and proteoglycans, which are components of the extracellular bone matrix, are also localized in and
at the membrane of osteoblasts and in the pericellular matrix. Due to their interaction with several growth factors, water
and cations these molecules play an important role in regulating proliferation and differentiation of osteoblasts and bone
development. The aim of this study was to assess in vitro the effects of two chemically sulfated hyaluronan (HyaS) derivatives on the proliferation of rat calvarial osteoblasts and
to compare with those of native hyaluronan (Hya) and natural sulfated GAG such as chondroitin-4-sulfate (C4S), chondroitin-6-sulfate
(C6S), dermatan sulfate (DS) and heparan sulfate (HS). Moderately and highly sulfated HyaS derivatives caused a time-dependent
reduction of osteoblast proliferation. The anti-proliferative effect of HyaS was accompanied by a cell cycle arrest in the
G1 phase, but was not associated with cell death. Whereas non-sulfated high molecular weight (HMW)- and low molecular weight
(LMW)-Hya as well as C4S, C6S, DS and HS showed no effect on the cell proliferation. |
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