Thermal inducible expression of xylose isomerase and its performance in a hollow fiber bioreactor |
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| Authors: | Dominic W. S. Wong Linda N. H. Yee Carl A. Batt |
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| Affiliation: | (1) Department of Food Science, Cornell University, 14853 Ithaca, NY, U.S.A. |
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| Abstract: | Summary TheEscherichia coli xylose isomerase (EC 5.3.1.5) has been expressed under the control of a thermal inverting promotor system (att-nutL-p-att-N block) and its performance in a hollow fiber bioreactor measured. The conversion of xylose to xylulose was inversely proportional to the flow rate and the system operated up to 60°C. The maximum conversion efficiency observed was 19.05% at 55°C. |
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| Keywords: | Xylose isomerase Enzyme expression thermally inducible Hollow fiber bioreactor Escherichia coli |
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