Recovery of an alkaline proteinase from fermentation broth using flocculation for cell removal |
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Authors: | V Sitkey M Minárik P Michalík |
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Institution: | (1) LIKO Research Institute, Miletičova 23, P.O. Box 4, 820 06 Bratislava, Czech & Slovak Federative Republic |
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Abstract: | Summary Recovery of a bacterial alkaline proteinase after treatment of the fermentation broth with 16 different flocculating additives
has been investigated. The polyelectrolyte Sedipur TF 5 was the most effective at 150 ppm and pH 7.0–9.0, giving a 74% yield
of enzyme activity. |
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