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Recovery of an alkaline proteinase from fermentation broth using flocculation for cell removal
Authors:V Sitkey  M Minárik  P Michalík
Institution:(1) LIKO Research Institute, Miletičova 23, P.O. Box 4, 820 06 Bratislava, Czech & Slovak Federative Republic
Abstract:Summary Recovery of a bacterial alkaline proteinase after treatment of the fermentation broth with 16 different flocculating additives has been investigated. The polyelectrolyte Sedipur TF 5 was the most effective at 150 ppm and pH 7.0–9.0, giving a 74% yield of enzyme activity.
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