无载体固定化酵母细胞木薯淀粉质原料酒精连续发酵研究

以木薯粉糖化液为发酵底物,在总发酵体积(有效)为15L的悬浮床生物反应器中,对一株粟酒裂殖酵母变异株进行一级和二级连续发酵研究。结果表明,二级连续发酵系统可明显改善一级系统的不足,并取得了平均流加糖液浓度150g/L,发酵强度为97g/L.h,流出液酒精浓度727g/L,残糖浓度374g./L,总糖利用率达90%的较好结果;整个系统在连续一个月的运行中从未发现染菌现象,发酵操作稳定。

CONTINUOUS ETHANOL FERMENTATION USING TAPIOCA STARCH BY IMMOBILIZED YEAST CELL WITHOUT CARRIER

A widespread interest has been noted in continuous power ethanol fermentation utilizing systems with immobilized yeast cell through self flocculating. We studied continuous ethanol fermentation by a naturally flocculent strain of Schizosaccharomyces pombe and the performance of two stage continuous ethanol fermentation system using suspended bioreactors(total effective vulume 1 5?L)has been established: the high biomass levels achieved allow efficient ehtanol conversion(72 7?g/L, average), residual glucose(3 74?g/L)and high productivity(9 7?g/L.h) for 150?g/L glucose concentration in feed(tapioca syrup)with average 90% total conversion of substrate. The system was run steadily during a month research.