Phage-display libraries of murine and human antibody Fab fragments |
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| Authors: | Jan Engberg Peter Sejer Andersen Leif Kofoed Nielsen Morten Dziegiel Lene K Johansen Bjarne Albrechtsen |
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| Institution: | (1) Department of Biology, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen, Denmark;(2) Blodbanken KI2031, Department of Clinical Immunology, University Hospital, Blegdamsvej 9, DK-2100 Copenhagen, Denmark |
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| Abstract: | We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine
or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using
ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented.
The latter protocol includes a procedure for trypsin elution of bound phage. |
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| Keywords: | Assembly PCR Fab libraries phage display phagemid vectors recombinant antibodies |
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