Haemolytic factor in bovine seminal vesicle fluid

The haemolytic factor of bovine seminal fluid has a protein nature; it is present in the precipitate obtained by treating the fluid with alcohol-ether, ammonium sulphate and rivanol; it occurs in three out of five protein fractions obtained by chromatographic separation on Sephadex G 100.
of Enzymatic treatment of seminal vesicle fluid by pronase caused total inactivation of the haemolytic factor. Chymotrypsin caused considerable damage, while a number other enzymes did not affect the activity of the haemolytic factor.