Production of HBsAg by growth rate control with recombinantSaccharomyces
cerevisiae in fed-batch |
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Authors: | Man Bock Gu Kyung Hwan Jung Mahn Hoon Park Kwang Soon Shin Kyong Ho Kim |
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Institution: | (1) Fermentation Technology Lab., Mogam Biotechnology Research Institute, #341 Pojung-ri, Kusung-myun, 449-910 Yongin-kun, Kyonggi-do, Korea |
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Abstract: | Summary To maintain a constant specific growth rate for a recombinantS.cerevisiae in fed-batch, the medium feeding rate has been controlled with respect to the hourly calculated glucose uptake rate. The recombinant yeast producing HBsAg showed the exponential production trend in proportion to the exponential cell growth. Total cell yield in fed-batch was about 0.402 g cells/g glucose, and HBsAg was produced about ten times more than in batch. Decrease of growth rate by HBsAg produced was not shown. |
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