Identification of dihydroetorphine in biological fluids by gas chromatography-mass spectrometry |
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| Authors: | Yi Luo Jian-lin Feng Feng Liu Xu-ying Hu |
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| Affiliation: | 1. College of Pharmacy, Duksung Women''s University, Seoul, South Korea;2. National Institute of Food and Drug Safety Evaluation, Osong, South Korea;3. Innovative Drug Center, Duksung Women''s University, 33, Samyangro 144-gil, DobongGu, Seoul, South Korea;4. National Forensic Hospital, South Korea;5. Lush Plastic Surgery, Seoul, South Korea;1. Forensic Medical Sciences, Department of Health Science, University of Florence, Largo Brambilla 3, Florence 50134, Italy;2. Legal Medicine, Department of Health Surveillance and Bioethics, Università Cattolica del Sacro Cuore, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy;3. Area of Pathology, Department of Woman and Child Health and Public Health, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome 00147, Italy;4. Department of Translational Medicine and Surgery, Catholic University of Rome, Fondazione Policlinico Universitario A. Gemelli, IRCCS, Rome 00168, Italy |
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| Abstract: | A method for the monitoring of dihydroetorphine hydrochloride, a powerful anaesthetic and analgesic drug, in biological fluids was developed, involving GC-MS with multiple selected-ion monitoring. Dihydroetorphine was extracted from human blood and urine with dichloromethane and then derivatized with N-heptafluorobutyrylimidazole after having been concentrated to dryness. A dihydroetorphine monoheptafluorobutyl derivative was formed, which showed good behaviour in GC-MS with electron impact ionization. Its molecular ion, m/z 609, and its main fragments, m/z 576, 534, 522 and 508, were selected as the ions for identification owing to their relative peak intensities and characteristics. The target drug was identified based on its retention time, its selected multiple ions and their relative intensities. This method was successfully used for the detection of dihydroetorphine in blood and urine from a dihydroetorphine addict and a poisoned patient, respectively. |
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