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Sialyl-Tn antigen expression and O-linked GalNAc-Thr synthesis by Trypanosoma cruzi |
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| Authors: | Freire Teresa Robello Carlos Soulé Silvia Ferreira Fernando Osinaga Eduardo |
| Institution: | Depto. de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. |
| Abstract: | Most Trypanosoma cruzi O-glycans are linked to Thr/Ser residues via N-acetylglucosamine. We report that the mucin-type carcinoma-associated sialyl-Tn antigen (NeuAc-GalNAc-O-Ser/Thr) is expressed by T. cruzi. A specific MAb allowed us to localize the antigen on the surface of epimastigotes and to identify reactive components in parasite lysates (32, 60, and 94kDa). In addition, ppGalNAc-T activity was characterized in epimastigotes, and direct evidence was obtained for the in vitro incorporation of GalNAc to a synthetic peptide derived from a T. cruzi mucin. These results add an as yet unknown complexity to the pathways of O-glycan biosynthesis in this protozoan parasite. |
| Keywords: | O-glycosylation Sialyl-Tn antigen d-galactosamine:polypeptide N-acetylgalactosaminyltransferase" target="_blank">UDP-N-acetyl-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase Trypanosoma cruzi |
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