Characterization of somatic Ca2+ clearance mechanisms in young and mature hippocampal granule cells |
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| Authors: | Sang Hun Lee Won-Kyung Ho Suk-Ho Lee |
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| Affiliation: | 1. Faculty of Natural Sciences, Lausitz University of Applied Sciences, Senftenberg, Großenhainer Str. 67, Germany;2. GA Generic Assays GmbH, Dahlewitz/Berlin, Germany;3. Institute of Liver Studies, Division of Transplantation Immunology and Mucosal Biology, King''s College London School of Medicine at King''s College Hospital, London, UK;4. Institute of Molecular and Clinical Immunology, Otto-von-Guericke-University, Magdeburg, Germany;5. Institute of Immunology, Technical University Dresden, Dresden, Germany;1. Department of Health Sciences, School of Medicine, University “A. Avogadro”, Via Solaroli, 17-28100 Novara, Italy;2. Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Preoperative and Pain Medicine, Brigham and Women''s Hospital and Harvard Medical School, 77 Louis Pasteur Avenue, Boston, MA 02115, USA;3. Nicox Research Institute, Via L. Ariosto, 20091 Bresso, Milano, Italy;4. The William Harvey Research Institute, Barts and The London Medical School, Charterhouse Square, London EC1M 6QB, UK;5. Interdisciplinary Research Centre of Autoimmune Diseases (IRCAD), Novara, Italy |
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| Abstract: | Calcium is a key regulator for expression of genes relevant to survival and maturation of newborn neurons. Mammalian hippocampal dentate gyrus generates new granule cells (GCs) throughout adult life. We identified young and mature GCs in hippocampi of young adult mice according to their electrical properties, and investigated contributions of Na/Ca exchanger (NCX), sarco-endoplasmic reticulum Ca2+-ATPase (SERCA), plasma membrane Ca2+-ATPase (PMCA) and mitochondria to Ca2+ clearance in somata of GCs. Somatic Ca2+ clearance was increased by about 50% as GCs matured. NCX activity increased proportionally during maturation with its relative contribution kept about 40% both in young and mature GCs. On the other hand, the developmental increases in activities of mitochondria and SERCA resulted in higher contributions to Ca2+ clearance in mature GCs than in young GCs. Especially mitochondrial function was most highly enhanced during maturation. PMCA activity, however, did not increase during maturation. Low Ca2+ clearance in immature GCs might facilitate higher Ca2+ accumulation during network activity, which in turn help survival of young GCs. |
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