Effects of glutathione on amyloid fibrillation of hen egg-white lysozyme |
| |
Authors: | Steven S.-S. Wang Shang-Wei Chou Kuan-Nan Liu Chia-Hung Wu |
| |
Affiliation: | Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan |
| |
Abstract: | This study examined the effects of glutathione on the fibrillation of hen egg-white lysozyme. We found that the fibrillation of lysozyme was considerably reduced by GSH while no anti-aggregating activity was detected with only GSSG. SDS-PAGE results also revealed that the addition of GSH led to an early occurrence of prominent lysozyme hydrolysis. Moreover, GSH was effective in inhibiting lysozyme fibrillation when GSH was added within 6 days of incubation. We conclude that the attenuation of lysozyme fibrillation is strongly dependent upon the redox environment. Our data may contribute to decipher the molecular mechanism of amyloid fibrillation. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|