The influence of thermal treatment and operational conditions on xanthan produced by X. arboricola pv pruni strain 106 |
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Authors: | Caroline D. Borges Regina C.M. de Paula Judith P.A. Feitosa Claire T. Vendruscolo |
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Affiliation: | 1. Centro de Biotecnologia, Universidade Federal de Pelotas, CP 354, CEP 96010-900, Pelotas, RS, Brazil;2. Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, CP 6021, CEP 60455-760, Fortaleza, CE, Brazil |
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Abstract: | The influence of thermal treatment and operational conditions (pH and stirrer speed) used in the process of xanthan production by Xanthomonas arboricola pv pruni strain 106 were evaluated through yield of xanthan, aqueous solution and fermentation broth viscosity, sodium content, pyruvate and acetyl content and molar mass. Different conditions used during the fermentation affected the xanthan characteristics. Thermal treatment decreased the final yield and pyruvate and acetyl content, and increased the xanthan aqueous solution and fermentation broth viscosities, as well as molar mass. In this study the best combination of yield and viscosity was obtained with the use of pH 7 and 400 rpm during fermentation and post-fermentation thermal treatment. Aggregation of xanthan molecules promoted by heating and detected through an increase of molar mass was apparently affected by the sodium content. As a result, a correlation between molar mass and xanthan solution viscosity could be observed. |
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