DNA probes using fluorescence resonance energy transfer (FRET): designs and applications. |
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Authors: | V V Didenko |
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Affiliation: | Baylor College of Medicine, Houston, TX, USA. vdidenko@bcm.tmc.edu |
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Abstract: | Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes. They are especially advantageous for the real-time monitoring of biochemical reactions and in vivo studies. This paper reviews the design and applications of various DNA-based probes that use FRET The approaches used in the design of new DNA FRET probes are discussed. |
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