Cloning of trichosanthin cDNA and its expression in Escherichia coli |
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| Authors: | P C Shaw M H Yung R H Zhu W K Ho T B Ng H W Yeung |
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| Affiliation: | Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T. |
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| Abstract: | Several cDNA clones coding for trichosanthin (TCS) have been isolated from a cDNA library prepared from the poly(A)+RNA of the root tuber of Trichosanthes kirilowii Maximowicz. The nucleotide sequence codes for a protein of 289 amino acids (aa) including a putative signal peptide of 23 aa and an extra 19 aa at the C terminus; the latter two have not been found in TCS obtained from the natural product [Collins et al., J. Biol. Chem. 265 (1990) 8665-8669]. Recombinant TCS (reTCS) was synthesized in Escherichia coli, in which the cDNA without the signal sequence was expressed under the control of the trc promoter; reTCS was detected by a rabbit anti-TCS antiserum. |
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