Loss of callose in the stigma papillae does not affect the Brassica self-incompatibility phenotype |
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Authors: | Waheeda Sulaman MaryAnne Arnoldo Kangfu Yu Lomas Tulsieram Steven J. Rothstein Daphne R. Goring |
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Affiliation: | (1) Biology Department, York University, 4700 Keele Street, North York, Ontario, Canada M3J 1P3, CA;(2) Pioneer Hi-Bred Production Ltd., Canola Research Station, Georgetown, Ontario, Canada L7G 4S7, CA;(3) Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario, Canada N1G 2W1, CA |
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Abstract: | As part of the Brassicaceae self-incompatibility response, callose is deposited in the stigma papillar cells. To determine if callose plays an important role in the rejection of incompatible pollen by the stigma, transgenic Brassica napus. L. plants were produced which express the tobacco β-1,3-glucanase cDNA (the enzyme which degrades callose) in the stigma papillae. Using aniline blue fluorescence, little or no callose was detected in the papillar cells of transgenic stigmas. However, the self-incompatibility system appeared to be unaffected based on the lack of pollen tube growth and the subsequent lack of seed set. The transgene had no effect on compatible pollinations. Thus, while callose deposition is associated with the B. napus self-incompatibility response, it is not required for the rejection of incompatible pollen. Received: 14 March 1997 / Accepted: 15 April 1997 |
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Keywords: | :Brassica (callose) Callose Self-incompati- bility response Pollen Stigma |
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