Residual stability of lipase from Candida rugosain hexane, supercritical CO , and supercritical SF |
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Authors: | F.-J. Kao S.A. Ekhorutomwen S.P. Sawan |
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Affiliation: | (1) Department of Chemistry, University of Massachusetts Lowell, One University Avenue, Lowell, Massachusetts, 01854, U.S.A |
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Abstract: | Lipase from Candida rugosa (EC 3.1.1.3) lost only 15% of its activity when held in supercritical CO and about 10% activity in both supercritical SF and hexane even after two days of incubation at up to 60°C and 82 atm.A pressure of 680 atm resulted in up to 15% loss of enzyme activity in supercritical CO and only about 5% loss of activity in supercritical SF 6 even at 410 atm. There was about 60% decrease in enzyme activity even at 1% water content in supercritical CO . Supercritical SF is a better solvent than supercritical CO and hexane. |
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