Molecular evidence of Plesiomonas shigelloides as a possible zoonotic agent |
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| Authors: | Carlos González-Rey Anja Siitonen Alona Pavlova Ivan Ciznar Stefan B. Svenson Karel Krovacek |
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| Affiliation: | 1.Section of Bacteriology, Faculty of Veterinary Medicine,Swedish University of Agricultural Sciences,Uppsala,Sweden;2.Bacteriology Unit, National Institute for Health and Welfare (THL),Helsinki,Finland;3.Department of Laboratory Medicine,Karolinska Institute,Stockholm,Sweden;4.Faculty of Public Health,Slovak Medical University,Bratislava,Slovakia;5.Department of Biomedical Sciences and Veterinary Public Health,Swedish University of Agricultural Sciences,Uppsala,Sweden;6.Faculty of Medicine,University of Ostrava,Ostrava,Czech Republic;7.QC-Microbiology, Sobi AB,Stockholm,Sweden;8.Sobi AB,Stockholm,Sweden |
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| Abstract: | The most frequently used method for establishing epidemiological relationships between Plesiomonas shigelloides strains is O:H serotyping. However, a number of strains are not serotypeable and isolates from diverse sources can display the same serovar. Moreover, since the zoonotic nature of Plesiomonas has been suggested and this hypothesis is based on the identical serovars found in animals and humans, we intend to use four DNA-based techniques: random amplified polymorphic DNA-PCR, enterobacterial repetitive intergenic consensus-PCR, repetitive extragenic palindromic-PCR, and pulsed field gel electrophoresis in order to screen 24 strains belonging to nine O:H serovars isolated from humans, animals, and the environment. In general, P. shigelloides showed a high genetic heterogeneity. Three pairs of strains, each containing a human and an animal isolate, displayed similar genotypes. This is the first report that provides molecular evidence that P. shigelloides may be zoonotic. |
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