TMBP200, a XMAP215 homologue of tobacco BY-2 cells,has an essential role in plant mitosis |
| |
| Authors: | Email author" target="_blank">Hiroki?YasuharaEmail author Yuki?Oe |
| |
| Institution: | (1) Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita Osaka, 564-8680, Japan;(2) Present address: Department of Neuroscience, Graduate School of Frontier Biosciences, Osaka University, Suita Osaka, 565-0871, Japan |
| |
| Abstract: | TMBP200 from tobacco BY-2 cells is a member of the highly conserved family of microtubule-associated proteins that includes
Xenopus XMAP215, human TOGp, and Arabidopsis MOR1/GEM1. XMAP215 homologues have an essential role in spindle assembly and function in animals and yeast, but their role
in plant mitosis is not fully clarified. Here, we show by immunoblot analysis that TMBP200 levels in synchronously cultured
BY-2 cells increased when the cells entered mitosis, thus indicating that TMBP200 plays an important role in mitosis in tobacco.
To investigate the role of TMBP200 in mitosis, we employed inducible RNA interference to silence TMBP200 expression in BY-2
cells. The resulting depletion of TMBP200 caused severe defects in bipolar spindle formation and resulted in the appearance
of multinucleated cells with variable-sized nuclei. This finding indicates that TMBP200 has an essential role in bipolar spindle
formation and function. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
