降解鸡毛真菌板蜡蚧的鉴定及产酶培养优化

鉴定降解鸡毛真菌并通过单因素和正交实验优化其产角蛋白酶发酵培养条件.从加入鸡毛粉钓饵的医院花坛土中分离筛选获得3株角蛋白高效降解真菌,利用形态学和分子系统学鉴定均为板蜡蚧(Lecani-cillium testudineum).单因素实验表明,对优选菌株1Y2-12产酶能力具促进作用的碳源为乳糖,氮源为酵母膏,无机离子...

Identification of Chicken Feather Degradable Fungus Lecanicillium testudineum and Its Cultural Optimization of Keratinase Activity

Chicken feather degradable fungi were identified and their fermentation conditions of the strains with high keratinase activity were optimized by single-factor and orthogonal experiments. Chicken feather powder was adopted to bait the keratin-degradable fungi from soil in a hospital parterre, three efficient keratin degradable fungal strains were isolated. The strains were all identified as Lecanicillium testudineum adopting morphology and molecular genealogy. Single factor experiment showed that C source that could promote enzyme producing ability of an optimized strain 1Y2-12 was lactose, N source was yeast extract, and inorganic ion was Zn²⁺. Orthogonal experiment showed that the affecting enzyme-producing activity of 1Y2-12 successively were N source > inorganic ion > C source; and the best combination to promote enzyme activity addition of N source was 0.15%, C source 2%, and inorganic ion 0.01%. Under this optimized conditions, the enzyme producing activity of strain 1Y2-12 reached up to 22.03 U/mL, 2.1 times of that before optimized. The results of the study showed that the genus of Lecanicillium could degrade chicken feather keratin, and the three identified strains were new record to China.