The effect of phenylacetic acid on ethylene formation in wheat seedlings |
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Authors: | Ivana Machá?kova L Chvojka V Na?inec Z Zmrhal |
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Institution: | (1) Department of Plant Nutrition, Research Institute of Crop Production, Drnovská 507, 161 06 Praha 6, Czechoslovakia;(2) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Ke dvoru 16, 166 30 Praha 6, Czechoslovakia |
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Abstract: | Phenylacetic acid (PAA) was found to induce ethylene formation in wheat coleoptile segments. In its most effective concentration
(0.5 mM) PAA was by approximately 60 % less active than 0.1 mM indole-3-acetic acid (IAA). PAA-induced ethylene formation
was stimulated with 0.1 mM L-methionine by 24 % and totally inhibited by 2.5 and 5 μ gml-1 aminoethoxyvinylglycin (AVG) and 10 μg ml-1 cycloheximide. Cyoloheximide in lower concentration (5 μg ml-1) and actinomycin D (10 μg ml-1) inhibited PAA-induced ethylene formation by 50 % and 40 %, respectively.
After the simultaneous addition of PAA and IAA ethylene formation was by 35 % lower than in the presence of IAA itself. Further,
the coleoptile segments preincubated in IAA and then incubated in PAA solution produced by 35 % less ethylene than those incubated
in plain buffer after preincubation in IAA. Quite the opposite effect was found when the segments were preincubated in PAA
and then transferred into IAA solution. This treatment resulted in 70 % stimulation of ethylene formation over segments preincubated
in PAA and incubated in buffer. |
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