Initiation and culture of potato tuber callus tissue with picloram |
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Authors: | Steven R Hagen Duane LeTourneau Paul Muneta Janice Brown |
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Institution: | (1) Food Research Center, University of Idaho, 83843 Moscow, ID, USA |
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Abstract: | Callus cultures of 7 potato cultivars were initiated from tuber tissue and maintained on Gelrite-solidified media with 1–20 M picloram as the only PGR. Ten M picloram was the optimal concentration for callus induction. By 4–6 weeks after explanting, there was sufficient callus produced for subculture to maintenance media which contained 1–20 M picloram as the only PGR. When grown in the dark at 25°C, subcultured callus typically increased 10-fold in wet weight in 4–5 weeks. The callus produced was friable and a light grey to cream color. Callus cultures were used to establish cell suspension cultures. Callus and cell suspension cultures have been maintained for over 2 years on the picloram containing media.Abbreviations BA
benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- MS
Murashige-Skoog
- NAA
naphthaleneacetic acid
- PGR
plant growth regulator
Research paper #9053 of the Idaho Agricultural Experiment Station. |
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Keywords: | callus Gelrite picloram potato tuber Solanum tuberosum L suspension cultures |
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