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Typing Tuber melanosporum and Chinese black truffle species by molecular markers |
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| Authors: | Francesco Paolocci rea Rubini Bruno Granetti Sergio Arcioni |
| Affiliation: | Laboratório de Fisiologia Celular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, Bl. G, Cidade Universitária, Rio de Janeiro, RJ 21941-590, Brazil;Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, SP 04023-062, Brazil |
| Abstract: | Morphologically similar species such as Chinese black truffles and Tuber melanosporum were typed by restriction length polymorphism of internal transcribed spacers. This analysis together with sequence comparison revealed the presence of high genetic variability among fruit bodies collected in China. Selection of primer pairs allowed the internal transcribed spacer region of both Chinese truffles and T. melanosporum to be specifically amplified. |
| Keywords: | Escherichia coli RAPD typing Clonal grouping Serotype |
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