Purification and Characterization of an Extracellular Cold-Active Serine Protease from the Psychrophilic Bacterium <Emphasis Type="Italic">Colwellia</Emphasis> sp. NJ341期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Purification and Characterization of an Extracellular Cold-Active Serine Protease from the Psychrophilic Bacterium Colwellia sp. NJ341

Authors:	Quan-Fu?Wang,Jin-Lai?Miao author-information" > author-information__contact u-icon-before" > mailto:miaojinlai@.com" title=" miaojinlai@.com" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Yan-Hua?Hou,Yu?Ding,Guo-Dong?Wang,Guang-You?Li

Affiliation:	(1) College of life Sciences, Ocean University of China, 266003 Qingdao, P.R. China;(2) Key Laboratory of Marine Bio-active Substances, First Institute of Oceanorgarphy, State Oceanic Administration, 266061 Qingdao, P.R. China;(3) Harbin Institute of Technology, 264209 Weihai, P.R. China

Abstract:	Colwellia sp. NJ341, isolated from Antarctic sea ice, secreted a cold-active serine protease. The purified protease had an apparent Mr of 60 kDa by SDS-PAGE and MALDI-TOF MS. It was active from pH 5–12 with maximum activity at 35 °C (assayed over 10 min). Activity at 0 °C was nearly 30% of the maximum activity. It was completely inhibited by phenylmethylsulfonyl fluoride.

Keywords:	Antarctic cold-active serine protease Colwellia psychrophilic bacteria
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