Histological Lesion of Spleen and Inhibition of Splenocyte Proliferation in Broilers Fed on Diets Excess in Selenium |
| |
Authors: | Xi Peng Hengmin Cui Junliang Deng Zhicai Zuo Weiming Lai |
| |
Institution: | (1) Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan, 625014, China; |
| |
Abstract: | The purpose of this 42-day study was to investigate the effects of excess dietary selenium on immune function by determining
morphological changes of spleen and cell cycle of splenocyte. Three hundred 1-day-old avian broilers were fed on a basic diet
(0.2 mg/kg selenium) or the same diet amended to contain 1, 5, 10, and 15 mg/kg selenium (Se) supplied as sodium selenite
(n = 60/group). Anatomically, the spleens were shrinked in volume with pallecent color. Histopathologically, lymphopenia in
splenic nodules and periarterial lymphatic sheaths and congestion of the red pulp were observed in 5, 10, and 15 mg/kg Se
group. By flow cytometry method, the percentage of G0/G1 phase splenocytes was significantly increased, whereas the percentages of S phase and G2+M phase splenocytes and the proliferation index were markedly decreased in 5, 10, and 15 mg/kg Se groups when compared with
those of 0.2 mg/kg group. The results confirmed that excess dietary Se as sodium selenite in the range of 5∼15 mg/kg caused
growth retardation of spleen by cell cycle blockage in young chickens. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|