Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of <Emphasis Type="Italic">Chryseobacterium gleum</Emphasis> |
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Authors: | Priti N Chaudhari Bhushan L Chaudhari Sudhir B Chincholkar |
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Institution: | (1) School of Life Sciences, North Maharashtra University, P B 80, Jalgaon, 425 001, India; |
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Abstract: | Cholesterol oxidase activity was studied during biotransformation of cholesterol to androsta-1,4-diene-3,17-dione (ADD) by
Chryseobacterium gleum. Spent LB media, containing cholesterol (3 mM≈1 g l−1) where the bacterium was grown for 24 h, at 30°C with constant shaking at 120 rpm, had the highest enzyme activity (167 U mg−1). The growing cells produced 0.076 g ADD from 1 g cholesterol l−1. |
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