Increased Apoptotic Lymphocyte Population in the Spleen of Young Chickens Fed on Diets High in Molybdenum |
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Authors: | Fan Yang Hengmin Cui Jie Xiao Xi Peng Junliang Deng Zhicai Zuo |
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Institution: | (1) College of Veterinary Medicine, Sichuan Agricutural University, Yaan, Sichuan, 625014, China; |
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Abstract: | The experiment was conducted with the objective of examining the effects of high molybdenum (Mo) on the apoptosis of splenic
lymphocytes in broilers by the methods of experimental pathology and flow cytometry (FCM). Three hundred 1-day-old avian broilers
were divided into four groups of 75 each and were fed on control diet (Mo 13 mg/kg) and high Mo diets (Mo 500 mg/kg, high
Mo group I; Mo 1,000 mg/kg, high Mo group II; and Mo 1,500 mg/kg, high Mo group III) for 42 days. The results showed that
the relative weight of spleen was decreased, and lymphocytes were histopathologically decreased in high Mo groups II and III.
Ultrastructurally, the apoptotic cells showed typical condensed nuclei with dark-round, petal-like, and horseshoe-like shapes.
The mitochondria were swelled, and the endoplasmic reticulum and the Golgi apparatus were dilated in high Mo groups II and
III. The statistical analyses by FCM indicated that the percentage of cellular apoptosis was higher in high Mo groups II and
III than in control group. Also, the TUNEL staining was consistent with the results of FCM. It was concluded that dietary
molybdenum in 1,000 and 1,500 mg/kg caused splenic lesions and lymphocyte apoptosis, which could inhibit the development of
spleen and could impair immune function in broilers. |
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