| 蕨菜黄酮苷元的组成及含量测定研究 |
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| 引用本文： | 陈乃东,陈乃富,陈存武,张莉,戴军. 蕨菜黄酮苷元的组成及含量测定研究[J]. 生物学杂志, 2013, 0(5): 33-36 |
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| 作者姓名： | 陈乃东 陈乃富 陈存武 张莉 戴军 |
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| 作者单位： | [1]皖西学院生物与制药工程学院,六安237012 [2]植物细胞工程安徽省工程技术研究中心,六安237012 |
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| 基金项目： | 国家自然科学基金（No.81274021）;安徽省自然科学基金（090413113）;安徽省高等学校省级自然科学研究重点项目（KJ2009A165,KJ2012A277）;安徽省高等学校省级自然科学研究一般项目（KJ20108259）;六安市定向委托皖西学院市级研究重点项目（2011LWA001） |
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| 摘 要： | 以80%乙醇浸提经乙醚脱脂预处理的干蕨菜粉,减压回收乙醇得浓缩液.浓缩液以8%硫酸沸水浴水解3 h后,加入20%NaOH中和至弱酸性,过滤,滤渣以三氯甲烷索氏提取获总蕨菜黄酮苷元.TLC法检测总黄酮苷元的组成,HPLC法测定其主要苷元的含量.结果表明,通过酸水解获得总蕨菜黄酮苷元,其主要成分为山奈酚,含量约占蕨菜干重的1.9%.
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| 关 键 词： | 蕨菜 黄酮 黄酮苷元 山奈酚 |
Study on the compositions and the content of flavonoid aglycones in Pteridium aquilinum |
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| CHEN Nai-dong,CHEN Nai-fu,CHEN Cun-wu,ZHANG Li,DAI Jun. Study on the compositions and the content of flavonoid aglycones in Pteridium aquilinum[J]. Journal of Biology, 2013, 0(5): 33-36 |
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| Authors: | CHEN Nai-dong CHEN Nai-fu CHEN Cun-wu ZHANG Li DAI Jun |
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| Affiliation: | 1. College of Bioteehnology and Pharmaceutical Engineering, West Anhui University; 2. Anhui Bioteehnology Research Center of Plant Cell Engineering, Lu＇an 237012, China) |
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| Abstract: | Dry Pteridium aquilinum powder was defatted with ether first, then extracted with 80% ethanol （in water）. The filter resi- due hydrolyzed from the extraction by 8% sulfuric acid and then neutralized by 10% NaOH was extracted with chloroform by soxhlet method to give flavonoid aglycones. The constituents and the contents of the aglycones were identified and determined by TLC and HPLC, respectively. The results showed that the flavonoid aglycones of Pteridium aquilinum could be obtained by acid hydrolysis. In the experiment, the main glycone of Pteridium aquilinum was kaempferol with total content I. 9%. |
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| Keywords: | Pteridium aquilinum flavonoids flavonoid aglycones kaempferol |
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