Gut-specific actinobacterial community structure and diversity associated with the wood-feeding termite species, Nasutitermes corniger (Motschulsky) described by nested PCR-DGGE analysis |
| |
Authors: | T Lefebvre E Miambi A Pando M Diouf C Rouland-Lefèvre |
| |
Institution: | 1. UMR 7618 Bioemco, Institut de Recherche pour le Développement, 32 avenue Henri Varagnat, 93143, Bondy Cedex, France 2. UMR 7618 Bioemco, Université Paris XII Val de Marne, 61 Avenue du Général de Gaulle, 94010, Créteil Cedex, France 3. IRD ?le de France, Laboratoire d’Ecologie des Sols Tropicaux, 32 avenue Henri Varagnat, 93143, Bondy Cedex, France
|
| |
Abstract: | This comprehensive survey studied the actinobacterial community structure and putative representative members associated with
the gut of the wood-feeding termite, Nasutitermes corniger (Motschulsky), using nested PCR-DGGE and 16S rDNA sequences analyses. The closest relatives of the actinobacteria inhabiting
the gut of Nasutitermes corniger were in five families, regardless of the geographical origin of the termite colony: Propionibacteriaceae, Streptomycetaceae, Cellulomonodaceae, Corynebacteriaceae and Rubrobacteraceae. Feeding termites on beech wood did not result in substantial changes in the actinobacterial community structure as revealed
by DGGE banding patterns. Most of the 16S rDNA sequences obtained after excision and sequencing of DGGE bands clustered with
those previously retrieved in termite guts. These results confirm the presence of gut-specific actinobacteria. Except for
the 16S rDNA sequences affiliated to Streptomycetaceae and Cellulomonodaceae, no sequence had more than 97% similarity with the closest isolated strains, indicating the presence of microorganisms that
have not yet been cultivated. These results suggest that members of the Actinomycetales order account for the largest proportion of the Actinobacteria phylum inhabiting the gut of the termite N. corniger. |
| |
Keywords: | Actinomycetes Termite gut Nested PCR DGGE |
本文献已被 SpringerLink 等数据库收录! |
|