Isolation of a respiratory-deficient Kluyveromyces fragilis mutant for the production of ethanol from Jerusalem artichoke |
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Authors: | Guiraud J P Bourgi J Stervinou M Claisse M Galzy P |
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Affiliation: | Laboratoire de Génétique et Microbiologie INRA-ENSA 34060 Montpellier Cedex, France. |
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Abstract: | A respiratory-deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especially from Jerusalem artichoke extracts containing large amounts of high polyfructosans ("early" extracts). |
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