Exposure of mammalian cells to 60-Hz magnetic or electric fields: analysis for DNA single-strand breaks |
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Authors: | J A Reese R F Jostes M E Frazier |
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Institution: | Biology and Chemistry Department, Pacific Northwest Laboratory, Richland, WA 99352. |
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Abstract: | Chinese hamster ovary (CHO) cells were exposed for 1 h to 60-Hz magnetic fields (0.1 or 2 mT), electric fields (1 or 38 V/m), or to combined magnetic and electric fields (2 mT and 38 V/m, respectively). Following exposure, the cells were lysed, and the DNA was analyzed for the presence of single-strand breaks (SSB), using the alkaline elution technique. No significant differences in numbers of DNA SSB were detected between exposed and sham-exposed cells. A positive control exposed to X-irradiation sustained SSB with a dose-related frequency. Cells exposed to nitrogen mustard (a known cross-linking agent) and X-irradiation demonstrated that the assay could detect cross-linked DNA under our conditions of electric and magnetic field exposures. |
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Keywords: | DNA damage X-irradiation cross-linked DNA ovary cells |
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