The N-terminal domain of the IP3 receptor gates store-operated hTrp3 channels. |
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Authors: | K Kiselyov G A Mignery M X Zhu S Muallem |
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Institution: | Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235, USA. |
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Abstract: | In the present work, we studied the interaction and effect of several IP3 receptor (IP3R) constructs on the gating of the store-operated (SOC) hTrp3 channel. Full-length IP3R coupled to silent hTrp3 channels in intact cells but did not activate them until stores were depleted of Ca2+. By contrast, constructs containing the IP3-binding domain activated silent hTrp3 channels in unstimulated cells and restored gating of hTrp3 by IP3 in excised plasma membrane patches. We conclude that the N-terminal domain of the IP3R functions as a gate and is sufficient for activation of SOCs. The sensing and transduction domains of the IP3R are required to maintain SOCs in an inactive state. |
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