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The structure of a putative exopolysaccharide of Burkholderia gladioli pv. agaricicola
Authors:Kaczynski Zbigniew  Karapetyan Gnuni  Evidente Antonio  Iacobellis Nicola S  Holst Otto
Institution:a Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, D-23845 Borstel, Germany
b Dipartimento di Scienze Del Suolo, Della Pianta e Dell’ Ambiente, Universitá Degli Studi di Napoli Federico II, I-80055 Portici (NA), Italy
c Dipartimento di Biologia, Difesa e Biotecnologie Agro Forestali, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
Abstract:A putative capsular polysaccharide containing d-rhamnose was isolated from the phytopathogenic bacterium Burkholderia gladioli pv. agaricicola by phenol/water extraction followed by ultracentrifugation of the separated water phase and gel-permeation chromatography of the thus obtained supernatant. By means of chemical analyses and NMR spectroscopy, the repeating unit of the polymer was shown to be a linear tetrasaccharide with the structure.Image
Keywords:Mushroom disease  Agaricus bitorquis  Burkholderia gladioli pv  agaricicola  Exopolysaccharide  NMR spectroscopy
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