Effect of selenite on glucocorticoid receptor |
| |
Authors: | Y Tashima M Terui H Itoh H Mizunuma R Kobayashi F Marumo |
| |
Affiliation: | Department of Biochemistry, School of Medicine, Akita University. |
| |
Abstract: | Rat liver glucocorticoid receptor was partially purified and characterized for its hormone binding using selenite. Selenite at very low concentrations irreversibly inhibited the hormone binding. The concentration for half maximal inhibition was approximately 2.8 microM. The inhibition was restored by dithiothreitol. The receptor-hormone complex became considerably insensitive to the selenite inhibition. The receptor inhibited by selenite was eluted at the same position as the native receptor from DEAE ion exchange and gel filtration columns. The results suggest that at least four sulfhydryl groups are located in the hormone binding domain of the receptor making a cluster. |
| |
Keywords: | |
|