| 枯草芽孢杆菌cdd基因敲除及对胞苷发酵的影响 |
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| 引用本文： | 苏静,黄静,谢希贤,徐庆阳,陈宁.枯草芽孢杆菌cdd基因敲除及对胞苷发酵的影响[J].生物技术通讯,2010,21(1):39-42. |
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| 作者姓名： | 苏静 黄静 谢希贤 徐庆阳 陈宁 |
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| 作者单位： | 天津科技大学,生物工程学院,工业微生物教育部重点实验室,天津,300457 |
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| 摘 要： | 目的:通过敲除出发菌株上的胞苷脱氨酶基因,阻断嘧啶代谢通量由胞苷流向尿苷和尿嘧啶,选育胞苷产生菌。方法:采用同源重组的方法敲除枯草芽孢杆菌TS8的胞苷脱氨酶基因cdd,并通过遗传稳定性实验验证其缺失标记和胞苷产量,通过摇瓶发酵实验对比出发菌株和缺失株的产苷水平。结果:cdd基因缺失菌株TSb发酵72h,发酵液中胞苷产量达到1.72g/L,与原始菌株相比提高了44.19%,且遗传性状稳定。结论:cdd基因的缺失可有效阻断嘧啶代谢通量由胞苷流向尿苷和尿嘧啶,提高胞苷产量。
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| 关 键 词： | 枯草芽孢杆菌 胞苷脱氨酶 同源重组 胞苷 |
Knockout of the cdd Gene in Bacillus subtilis and its Influence on Cytidine Fermentation |
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| SU Jing,HUANG Jing,XIE Xi-Xian,XU Qing-Yang,CHEN Ning.Knockout of the cdd Gene in Bacillus subtilis and its Influence on Cytidine Fermentation[J].Letters in Biotechnology,2010,21(1):39-42. |
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| Authors: | SU Jing HUANG Jing XIE Xi-Xian XU Qing-Yang CHEN Ning |
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| Institution: | SU Jing,HUANG Jing,XIE Xi-Xian,XU Qing-Yang,CHEN Ning College of Bioengineering,Key Laboratory of Industrial Microbiology of Ministry of Education,Tianjin University of Science&Technology,Tianjin 300457,China |
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| Abstract: | Objective:To knockout cytidine deaminase gene of original strain for blocking pyrimidine metabolic flux to flow from cytidine to uridine and uracil,and to select cytidine producing bacteria.Methods:The cytidine deaminase gene cdd of Bacillus subtilis TS8 was knockouted by using homologous recombination,its resistance and production were verified through stability experiment,and the level of cytidine production between mutant and original strain was compared.Results:The mutant B.subtilis TSb accumulated cyti... |
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| Keywords: | Bacillus subtilis cytidine deaminase homologous recombination cytidine |
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