| 过氧化物酶同工酶应用于甜瓜杂交种真实性与纯度检测研究 |
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| 引用本文： | 黄永红,董林阁.过氧化物酶同工酶应用于甜瓜杂交种真实性与纯度检测研究[J].生物技术,1993,3(6):21-27. |
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| 作者姓名： | 黄永红 董林阁 |
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| 作者单位： | 大庆师专生化系生物教研室 大庆
(黄永红,董林阁,韩明,王付德,王延波),大庆师专生化系生物教研室 大庆(杨建国) |
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| 摘 要： | 本研究利用聚丙烯酰胺凝胶电泳法分析了甜瓜(Cucumis melo.L)84—3等六个杂交种及其双亲的苗期过氧化物酶同工酶谱变化,按生长顺序在苗期进行了四次取样,每次取样均取全株。分析结果表明:甜瓜苗期生一片真叶时酶谱丰富,为取样最佳时期,同一组合中,F_1的过氧化物酶同工酶谱与亲本的过氧化物酶同工酶谱是有差异的,存在“杂种酶带”和“互补酶带”,利用过氧化物酶同工酶谱技术对甜瓜84-3等六个组合进行纯度检测结果与田间纯度检测结果相比符合率为99.1％。
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| 关 键 词： | 甜瓜 纯度 杂种 同功酶 |
Study of Authenticity and Purity Test in Cucumis melo L. Hybrids with Peroxidase Isozyme Technique |
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| Huang Yonghong Dong Lingo Han Ming,Wang Fude Wang Yanbo Yang Jianguo.Study of Authenticity and Purity Test in Cucumis melo L. Hybrids with Peroxidase Isozyme Technique[J].Biotechnology,1993,3(6):21-27. |
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| Authors: | Huang Yonghong Dong Lingo Han Ming Wang Fude Wang Yanbo Yang Jianguo |
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| Abstract: | This study has analysed peroxidase isozyme zymogram changes in seedl-ing of Cucumis melo L. 84-3 etc. 6 hydrids and their parents by polyac-rylamide gel electropnoresis. Samples are take 4 times in seedling accord-
ing to their growth stages. Whole plants are taken in each time sampling.
Analysis result showed that zymogram is rich rnd it's the best time to sample in the time of one euphylla. In the same group, there are discrepance on the
zymogram patterns of F1 generations and its parents, exist "hybrid band" and "complementary band" in the. F1 generationes. Comparing pcroxiclase iso-zyme purity test result with field's result, the corresponding rate is 99.1%. |
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| Keywords: | Cucumis me to L Hybrid Tsozyme Purity |
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