Bovine lens 23, 21 and 19 kDa intrinsic membrane proteins have an identical amino-terminal amino acid sequence |
| |
Authors: | Gadiparthi N Rao Karen A Gutekunst Robert L Church |
| |
Institution: | Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA 30322. |
| |
Abstract: | We have isolated bovine lens intrinsic membrane proteins (MP) having molecular masses of 19, 21 and 23 kDa. Limited amino acid sequence analysis of the amino-terminal portion of each of these polypeptides revealed a 100% homology in sequence for the number of residues determined (20 amino acids). Northern blot analysis of bovine lens mRNA using a labeled antisense oligonucleotide probe common to the amino acid sequence of these three peptides revealed a single band having an apparent molecular size of 0.8 kb. Taken together, these findings suggest a genetic commonality between these polypeptides. |
| |
Keywords: | Amino acid sequencing Lens membrane protein mRNA Synthetic antisense oligonucleotide |
本文献已被 ScienceDirect 等数据库收录! |