A previously reported polymorphic plasma protein of dogs and horses, identified as apolipoprotein A-IV |
| |
Authors: | R. K. JUNEJA ,B. GAHNE,M. LUKKA&dagger ,C. EHNHOLM&dagger |
| |
Affiliation: | *Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden;†National Public Health Institute, Helsinki, Finland |
| |
Abstract: | Summary. By using immunoblotting with antiserum specific to human plasma apolipoprotein A-IV (apoA-IV), a previously reported polymorphic plasma protein of dogs viz postalbumin-2 (Pa2) and one of horses viz serum protein 2 (SP2), were identified as apoA-IV of these species. This along with earlier published results implied that: (1) both dog and horse show a high degree of polymorphism at the APOA4 locus with three common alleles in each of the two species; and (2) apoA-IV phenotyping in these two species can be done by analysing plasma/serum samples by a simple method of two-dimensional electrophoresis, conducted under non-denaturing conditions, followed by general-protein staining of gels. |
| |
Keywords: | dog horse plasma apolipoprotein A-IV genetic polymorphism immunoblotting |
|
|